Reporter

Part:BBa_K4458010

Designed by: Beth Tuck   Group: iGEM22_East_Coast_BioCrew   (2022-09-10)


CYP1A1-CYC1-Akaluc

Provides a dioxin-responsive element (DRE), which when PCBs and other phenyl compounds are bound to aryl hydrocarbon receptor nuclear translocator (ARNT), induces expression of AkaLuc. AkaLuc is a modified version of luciferase that binds to luciferin substrate analog Akalumine-HCl.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 119
    Illegal NheI site found at 123
    Illegal NheI site found at 127
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None